![]() PCR products purified using the PureLink™ Pro 96 PCR Purification Kit demonstrate outstanding performance in downstream applications such as cloning automated fluorescent DNA sequencing, PCR, restriction mapping, and labeling. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform. The extent of the ABCA1-induced modication on the attributes of both the inner and outer membrane leaets was assessed by the evaluation, in transfected HeLa cells, of membrane translocation of cationic probes developed by Yeung et al. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. the membrane by novel biophysical techniques applied to living cells. Improved plate design The PureLink™ Pro 96 PCR Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. The simple PureLink™ bind/wash/elute procedure facilitates routine purifications of double-stranded DNA fragments from 100bp to 12kb. coli culture grown overnight in a square-well block, typical yields are 5-15g plasmid DNA, with an average of 9.4g. coli strains grown in up to 5mL LB, 3mL 2xYT, or 3mL TB. the addition of protein G Protein Magnetic Beads (Invitrogen, Waltham, MA. The PureLink™ Pro 96 PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products. The PureLink Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20g of plasmid DNA from E. Precleared The Thermo Scientific Pierce Protein A/G Magnetic Beads enable. The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
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